Deoxyribonuclease I from Worthington Biochemical Corporation

Product Specs
ItemDeoxyribonuclease I
CompanyWorthington Biochemical Corporation
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberLS002006
Source/Expression SystemBovine Pancreas
StorageStore at 2-8C. PROTECT FROM MOISTURE
Activity≥2,000 Kunitz units per mg dry weight
Quantity20 mg

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Prospective isolation of neural stem and progenitor cells from the developing human brainSTAR ProtocolsDecember 1, 2023Daniel Dan Liu, Joy Q. He, Nobuko Uchida, Irving L. Weissman, Rahul Sinhapeptides, and recombinant proteins" ,, Liberase,Roche,Cat#5401119001 DNase I,Worthington Biochemical,Cat#LS002007 Accutase,Innovative Cell Technologies,Cat#AT104 Polyvinyl alcohol,Sigma,Cat#P8136 Poly-L-ornithine

(2) Secreted antigen A peptidoglycan hydrolase is essential for Enterococcus faecium cell separation and priming of immune checkpoint inhibitor cancer therapybioRxivNovember 26, 2023Steven Klupt, Kyong Tkhe Fam, Xing Zhang, Pavan Kumar Chodisetti, Abeera Mehmood, Tumara Boyd, Danielle Grotjahn, Donghyun Park, Howard C. Hangin RPMI with 1.5 U mL−1 Liberase TM (Roche 5401119001), 0.2 mg mL-1 DNase I (Worthington Biochemical LS002006) and ceramic spheres (6.35 mm, MP Biomedicals 116540424-CF) for 30 min at 37 °C with gentle shaking.

(3) Generation of gastirc insulin-secreting organoids from human stomach sampleResearch squareApril 27, 2023Huang X, Zhou Q.Collagenase IV (Worthington Biochemical, LS004188) (optional) 2 U/mL DNase I (Worthington Biochemical, LS002007) hGSC medium 66.7% DMEM, high glucose (Thermo Fisher Scientific, 11-965-118) 33.3% F12K (Thermo Fisher

(4) Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse modelsSTAR ProtocolsMarch 1, 2023Apeng Chen, Yapeng Chao, Han Zou, Lauren H. McCarl, Gary Kohanbash, Baoli Hu,Worthington Biochemical Corporation,Cat# LS003587 Deoxyribonuclease I,Worthington Biochemical Corporation,Cat# LS002007 ACK lysing buffer,Gibco,Cat# A10492-01 Fetal bovine serum,Sigma-Aldrich,Cat# F0926 Dulbecco’s phosphate buffered saline

(5) Lymphangiocrine signals are required for proper intestinal repair after cytotoxic injuryCell stem cellAugust 4, 2022Palikuqi B, Rispal J, Reyes EA, Vaka D, Boffelli D, Klein O.A,Sigma-Aldrich,Cat# 10103586001 Dispase II,Sigma-Aldrich,Cat# 4942078001 DNAse,Worthington Biochemical,Cat# LS002007 iTaq™ Universal SYBR® Green Supermix,Biorad,Cat# 1725124 Lectin from Ulex europaeus (FITC conjugated),Sigma-Aldrich,Cat#

(6) Fresh and cryopreserved ovarian tissue from deceased young donors yields viable folliclesF&S ScienceFebruary 11, 2022Yuting Fan, Colleen L. Flanagan, Margaret A. Brunette, Andrea S. Jones, Brendon M. Baker, Sherman J. Silber, Ariella Shikanov(DPBS) with Ca2+ and Mg2+ (14040133, Gibco, Thermo Fisher Scientific, MA, USA) with 10 IU DNase I (LS002007, Worthington Biochemical Corp, NJ, USA) and 1.0 mg/ mL Collagenase IA (C2674, Sigma-Aldrich, MO, USA).

(7) Effects of the Selective Serotonin Reuptake Inhibitor Fluoxetine on Developing Neural Circuits in a Model of the Human Fetal CortexInternational journal of molecular sciencesSeptember 28, 2021Tate K, Kirk B, Tseng A, Ulffers A, Litwa K.(22.5 mM), NaHCO3 (26 mM), DNase (125 U/mL, Worthington Biochemical Corporation, Lakewood, NJ, USA, LS002007), papain (30 U/mL, Worthington LS03126), and L-cysteine (1 mM, Sigma, C7880). Post-incubation the cortical

(8) In vitro co-culture of human intestinal organoids and lamina propria-derived CD4⁺ T cellsSTAR protocolsJune 18, 2021Schreurs RRCE, Baumdick ME, Drewniak A, Bunders MJ.129830-38-2 Collagenase D,Roche,Cat# 11088866001; EC:3.4.24.3 DNase I,Worthington Biochemical Corporation,Cat# LS002007 Percoll (GE Healthcare),Sigma-Aldrich,Cat# GE17-0891-01 "1,4-Dithiothreitol (DTT)",Sigma-Aldrich,Cat#

(9) Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discoveryImmunityMarch 9, 2021Chiou SH, Tseng D, Reuben A, Mallajosyula V, Molina IS, Conley S, Wilhelmy J, McSween AM, Yang X, Nishimiya D, et al.Proteins" ,, Collagenase III,Worthington Biochemical,Cat#: LS004182 DNase I,Worthington Biochemical,Cat#: LS002007 Zombie Aqua,Biolegend,Cat#: 423102 Human TruStain FcX,Biolegend,Cat#: 422302 Live/dead near-IR dye,Thermo Fisher Scientific

(10) CNS fibroblasts form a fibrotic scar in response to immune cell infiltrationNature neuroscienceFebruary 1, 2021Dorrier CE, Aran D, Haenelt EA, Sheehy RN, Hoi KK, Pintarić L, Chen Y, Lizama CO, Cautivo KM, Weiner GA, et al.papain (Worthington Biochemical, LK003176, 1 vial per sample) containing DNase (125 U/ml, Worthington LS002007) for 1.5 hours at 35°C. This was followed by mechanical trituration in a solution containing ovomucoid

(11) Preparation, Assembly, and Transduction of Transgenic Elements Using Mosaic Analysis with Dual Recombinases (MADR)STAR ProtocolsDecember 30, 2020David Rincon Fernandez Pacheco, Sara Sabet, Joshua J. Breunig(bFGF),Millipore,Cat# GF003 Collagenase IV,Worthington Biochemical,Cat# LS004189 DNase I,Worthington Biochemical,Cat# LS002007 Neurobasal media,Thermo Fisher Scientific,Cat# 21103049 Trypsin inhibitor,N/A,N/A Penicillin-streptomycin-amphotericin,Thermo Fisher Scientific

(12) G9a Promotes Breast Cancer Recurrence through Repression of a Pro-inflammatory ProgramCell reportsNovember 3, 2020Mabe NW, Garcia NMG, Wolery SE, Newcomb R, Meingasner RC, Vilona BA, Lupo R, Lin CC, Chi JT, Alvarez JV.#11076 Polybrene,Sigma,Cat#107689 Puromycin,Sigma,Cat#P8833 Blasticidin,GIBCO,Cat#A11139–03 Collagenase / hyaluronidase,Stemcell,Cat#7912 Dispase,Stemcell,Cat#7913 DNase I,Worthington Biochemical,Cat#LS002006 FBS,Corning,Cat#35010CV Penicillin/Streptomycin,Gicbo,Cat#15140–122 Doxycycline,RPI,Cat#D43020 Critical

(13) MYCN drives chemoresistance in small cell lung cancer while USP7 inhibition can restore chemosensitivityGenes & DevelopmentSeptember 1, 2020Eli Grunblatt et al.followed by a 15-min digestion at 37°C in RPMI1640 containing 80 U/mL DNase (Worthington Biochemical LS002007) and 300 U/mL collagenase type 1 (Worthington Biochemical LS004196). Digested lungs were sheared through

(14) Generation of Mosaic Mammary Organoids by Differential TrypsinizationJournal of Visualized ExperimentsApril 1, 2020Stefany Rubio, Oscar Cazares, Hector Macias, Lindsay HinckScientific,CB-40230C,"Lot: 8204010, stock concentration 8.9 mg/mL" DNase (Deoxyribonuclease I),Worthington Biochemical,LS002007, "Millicell EZ SLIDE 8-well glass, sterile",Millipore Sigma,PEZGS0816,These chamber slides are great

(15) The loss of microglia activities facilitates glaucoma progression in association with CYP1B1 gene mutation (p.Gly61Glu)PloS oneJanuary 1, 2020Alghamdi A, Aldossary W, Albahkali S, Alotaibi B, Alrfaei BM.USA), 1% Pen-Strep antibiotic, DMEM, trypsin (Catalogue #12604–013, Gibco, USA), DNase (Catalogue #LS002007, Worthington Biochemical Corporation, USA) and PBS (Catalogue #10010–031, Gibco, USA). These tubes were

(16) CD301b/MGL2 ⁺ Mononuclear Phagocytes Orchestrate Autoimmune Cardiac Valve Inflammation and FibrosisCirculationJune 5, 2018Lee A. Meier, Jennifer L. Auger, Brianna J. Engelson, Hannah M. Cowan, Elise R. Breed, Mayra I. Gonzalez-Torres, Joshua D. Boyer, Mayank Verma, Aubyn Marath, Bryce A. Binstadt500 U/mL collagenase-2 (Worthington Biochemical, LS004174), 20 U/mL DNase I (Worthington Biochemical, LS002006). Samples were incubated at 37°C using a water bath with periodic trituration for 1 hour total enzyme

(17) Generation and use of a humanized bone-marrow-ossicle niche for hematopoietic xenotransplantation into miceNature protocolsOctober 1, 2017Reinisch A, Hernandez DC, Schallmoser K, Majeti R.Aldrich, cat. no. 31392) DNase (Deoxyribonuclease I, Worthington Biochemical Corporation, cat. no. LS002007) Ultra pure 0.5 M EDTA (Fisher, cat.no. 155-75-020) Fetal bovine serum (FBS, Omega Scientific, individual

(18) G-actin provides substrate-specificity to eukaryotic initiation factor 2α holophosphataseseLifeMarch 16, 2015Chen R, Rato C, Yan Y, Crespillo-Casado A, Clarke HJ, Harding HP, Marciniak SJ, Read RJ, Ron D.bovine pancreatic DNase I (>2000 units/mg) was purchased from Worthington Biochemical Corporation (#LS002006, Lakewood NJ) and constituted into a complex with actin as previously described (Mannherz et al., 1980). Binary

(19) Purification of Oligodendrocyte Precursor Cells from Rat Cortices by ImmunopanningCold Spring Harbor ProtocolsAugust 1, 2013Jason C. Dugas, Ben EmeryLaboratory Press J.C. Dugas and B. Emery On ice, dissolve 12,500 U of DNase I (Worthington Biochemical LS002007) per 1 mL of chilled Earle’s Balanced Salt Solution (EBSS; Invitrogen 14155-063). Filter-sterilize on

(20) Immune clearance of attenuated rabies virus results in neuronal survival with altered gene expressionPLoS pathogensJanuary 1, 2012Gomme EA, Wirblich C, Addya S, Rall GF, Schnell MJ.was incubated in 20 units/mL preactivated papain and 125 units/mL DNase I (Worthington Biochemical, LS002006) for 85 min in a 37°C water bath, triturating gently with a 10 mL pipette twice throughout the incubation.

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Deoxyribonuclease I from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Deoxyribonuclease I from Worthington Biochemical Corporation

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